BCG vaccination in the cotton rat (Sigmodon hispidus) infected by the pulmonary route with virulent Mycobacterium tuberculosis.

abstract

• To evaluate the usefulness of the American cotton rat (Sigmodon hispidus) in the evaluation of vaccine-induced resistance, we infected BCG-vaccinated and non-vaccinated cotton rats with Mycobacterium tuberculosis (H37Rv) via the respiratory route. Lung histopathology of these animals showed loose, disorganized granulomas which were non-necrotic up to 8 weeks post-infection. Moreover, we were not able to detect a DTH response after intradermal injection with PPD antigen. Prior BCG vaccination significantly reduced lung and spleen bacterial loads by 1-1.5log CFU and upregulated PPD-induced proliferation and production of IFNgamma in lymphocyte cultures. We conclude that pulmonary infection of the cotton rat with Mtb more closely resembles the phenotype seen in mice rather than guinea pigs.

authors

• Weeks, Bradley

published proceedings

• Tuberculosis (Edinb)

author list (cited authors)

• McFarland, C. T., Ly, L., Jeevan, A., Yamamoto, T., Weeks, B., Izzo, A., & McMurray, D.

citation count

• 7

complete list of authors

• McFarland, Christine T||Ly, Lan||Jeevan, Amminikutty||Yamamoto, Toshiko||Weeks, Bradley||Izzo, Angelo||McMurray, David

publication date

• January 2010

publisher

• Elsevier  Publisher

published in

• Tuberculosis  Journal

keywords

• Administration, Inhalation
• Aerosols
• Animals
• BCG Vaccine
• Bacterial Load
• Cell Proliferation
• Cells, Cultured
• Disease Models, Animal
• Female
• Granuloma
• Hypersensitivity, Delayed
• Immunity, Cellular
• Interferon-gamma
• Lung
• Male
• Mycobacterium Tuberculosis
• Sigmodontinae
• Spleen
• Tuberculin
• Tuberculin Test
• Tuberculosis, Pulmonary
• Vaccination
• Virulence

Digital Object Identifier (DOI)

• 10.1016/j.tube.2010.03.014

start page

• 262

end page

• 267

volume

• 90

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.tube.2010.03.014

user-defined tag

• 3 Good Health and Well-Being